The dimer content of active ingredients of antioxidant TMQ was analysis by quantitative analysis of high performance liquid chromatography（HPLC） method in GB/T 8826—2011，qualitative analysis of infrared spectroscopy，and semi-quantitative analysis of thin layer chromatography（TLC）.The results showed that，the quantitative analysis of dimer content of active ingredients of antioxidant TMQ by HPLC，which used the area normalization method for data processing had certain limitations.It was recommended to use the internal standard method or external standard method for quantitative analysis.For doubtful analysis results，the results could be analyzed by combination of HPLC and TLC.
